Milla Jovovich as Alice

Michelle Rodriguez as Rain Ocampo

Eric Mabius as Matt Addison

James Purefoy as Spence Parks

Martin Crewes as Chad Kaplan

Colin Salmon as James "One" Shade

Pasquale Aleardi as J.D. Salinas

Heike Makatsch as Dr. Lisa Addison

Ryan McCluskey as Mr. Grey

Indra Ové as Ms. Black

Oscar Pearce as Mr. Red

Anna Bolt as Dr. Green

Joseph May as Dr. Blue

Robert Tannion as Dr. Brown

Jaymes Butler as Clarence

Stephen Billington as Mr. White

Fiona Glascott as Ms. Gold

Liz May Brice as Medic

Torsten Jerabek as Commando 1

Marc Logan-Black as Commando 2

Michaela Dicker as Red Queen

Jason Isaacs as Dr. William Birkin (uncredited)