Zach Braff as Chicken Little (voice)

Garry Marshall as Buck Cluck (voice)

Don Knotts as Mayor Turkey Lurkey (voice)

Amy Sedaris as Foxy Loxy (voice)

Steve Zahn as Runt of the Litter (voice)

Joan Cusack as Abby Mallard (voice)

Patrick Stewart as Mr. Woolensworth (voice)

Fred Willard as Melvin - Alien Dad (voice)

Catherine O'Hara as Tina - Alien Mom (voice)

Wallace Shawn as Principal Fetchit (voice)

Harry Shearer as Dog Announcer (voice)

Mark Walton as Goosey Loosey (voice)

Sean Elmore as Kirby - Alien Kid (voice)

Matthew Josten as Kirby - Alien Kid (voice)

Evan Dunn as Kirby - Alien Kid (voice)

Mark Dindal as Morkubine Porcupine / Coach (voice)

Dan Molina as Fish Out of Water (voice)

Patrick Warburton as Alien Cop (voice)

Adam West as Ace - Hollywood Chicken Little (voice)

Joe Whyte as Rodriguez / Acorn Mascot / Umpire (voice)

Kelly Hoover as Mama Runt (voice)

Will Finn as Hollywood Fish (voice)

Dara McGarry as Hollywood Abby (voice)

Mark Kennedy as Hollywood Runt (voice)

Terri Douglas as Additional Voices (voice)

Pat Fraley as Additional Voices (voice)

Scott Menville as Additional Voices (voice)

Aaron Spann as Additional Voices (voice)

Nathan Kress as Additional Voices (voice)

Brittney Lee Harvey as Additional Voices (voice)

Chris Edgerly as Additional Voices (voice)

Julianne Buescher as Additional Voices (voice)

Mona Marshall as Additional Voices (voice)

Eddie Frierson as Additional Voices (voice)