Michael J. Fox as Stuart Little (voice)

Melanie Griffith as Margalo (voice)

Nathan Lane as Snowbell (voice)

Geena Davis as Mrs. Little

Hugh Laurie as Mr. Little

Jonathan Lipnicki as George Little

Steve Zahn as Monty (voice)

James Woods as Falcon (voice)

Maria Bamford as Teacher

Anna Hoelck as Martha Little

Ashley Hoelck as Martha Little

Kevin Olson as Irwin

Marc John Jefferies as Will

Michael C. Fuchs as Mark

Frank Aquilino as Onlooker

Angelo Massagli as Wallace

Brad Garrett as Plumber

Conan McCarty as Referee

Jim Doughan as Soccerball Coach

Rachael Harris as Additional Voices (voice)