Kate Higgins as Frankie Stein / Lilith Van Helscream (voice)

Cindy Robinson as Jackson Jekyll / Holt Hyde / Operetta / Additional Voice (voice)

Salli Saffioti as Clawdeen Wolf / Cleo de Nile (voice)

Erin Fitzgerald as Abbey Bominable / C.A. Cupid / Clair / Additional Voices (voice)

Laura Bailey as Lagoona Blue / Headmistress Bloodgood (voice)

Debi Derryberry as Draculaura (voice)

Ogie Banks as Clawd Wolf (voice)

Evan Smith as Deuce Gorgon / Gil Webber / Thad (voice)

America Young as Howleen Wolf / Toralei Stripe (voice)

Audu Paden as Ghoulia Yelps / Manny Taur / Sheriff / Additional voices (voice)

Cam Clarke as Heath Burns / Mr. Rotter / Goon #2 (voice)

Julie Maddalena as Robecca Steam / Venus McFlytrap (voice)

Steve Staley as Chad / Kid #1 (voice)

Mark Mercado as Gargoyle (voice)

Ira Singerman as Goon #1 (voice)

Andrew Duncan as Narrator (voice)

Wendee Lee as Nefera De Nile (voice) (uncredited)

Jonathan Lipow as Tiki People (voice) (uncredited)