Peter Weller as Batman / Bruce Wayne (voice)

Ariel Winter as Robin / Carrie Kelley (voice)

David Selby as James Gordon (voice)

Wade Williams as Harvey Dent / Two-Face (voice)

Michael McKean as Dr. Bartholomew Wolper (voice)

Rob Paulsen as Jeff Adams (voice)

Gary Anthony Williams as Mutant Leader / Anchor Bill (voice)

Gary Anthony Sturgis as Silk / Other Spike / Young Mutant (voice)

Paget Brewster as Lana Lang (voice)

Carlos Alazraqui as Hernando / TV Host (voice)

Dee Bradley Baker as Don / Fat Mutant / Additional Voices (voice)

Maria Canals-Barrera as Yindel (voice)

Cathy Cavadini as Joanie / Woman with Hot Dog (voice)

Townsend Coleman as Morrie / Civilian (voice)

Grey DeLisle as Anchor Carla / Female Mutant (voice)

Greg Eagles as Mackie (voice)

Michael Emerson as Joker (voice)

Michael Jackson as Alfred Pennyworth (voice)

Maurice LaMarche as Dr. Willing / News Anchor (voice)

Sam McMurray as Anchor Ted (voice)

Andrea Romano as Woman / Police Dispatch (voice)

Tara Strong as Michelle / Young Bruce Wayne / Kevin Ridley / Anchor Trish / Additional Voices (voice)

James Patrick Stuart as Murray (voice)

James Arnold Taylor as Mr. Hudson / Spud / Additional Voices (voice)

Bruce Timm as Thomas Wayne (voice)

Gwendoline Yeo as Lola Chong (voice)

Danny Jacobs as Merkel (voice)

Yuri Lowenthal as Son of Batman / Rookie Police Officer (voice)

Richard Doyle as The Mayor (voice)

Frank Welker as Mayor Stevenson / Weather Man (voice)

Jim Wise as Femur (voice)