Jason O'Mara as Batman / Bruce Wayne (voice)

Jennifer Morrison as Catwoman / Selina Kyle (voice)

Maury Sterling as Thomas Elliot (voice)

Sean Maher as Nightwing / Dick Grayson (voice)

Geoffrey Arend as The Riddler / Edward Nigma (voice)

James Garrett as Alfred Pennyworth (voice)

Jerry O'Connell as Superman / Kal-El / Clark Kent (voice)

Rebecca Romijn as Lois Lane (voice)

Bruce Thomas as Jim Gordon (voice)

Rainn Wilson as Lex Luthor (voice)

Peyton List as Batgirl / Barbara Gordon (voice)

Stuart Allan as Robin / Damian Wayne (voice)

Vanessa Williams as Amanda Waller (voice)

Peyton List as Poison Ivy / Pamela Isley (voice)

Jason Spisak as The Joker (voice)

Hynden Walch as Harley Quinn (voice)

Adam Gifford as Bane / Clayface (voice)

Sachie Alessio as Lady Shiva (voice)

Chris Cox as Scarecrow (voice)

Tara Strong as Reporter (voice)